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Erastin

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Erastin

Erastin 是鐵誘導(dǎo)的細(xì)胞死亡 (ferroptosis) 誘導(dǎo)劑。Erastin 結(jié)合且抑制電壓依賴性陰離子通道 (VDAC2/VDAC3)。
貨號： HY-15763

參數(shù)品牌：MCE

產(chǎn)品參數(shù)

品牌：MCE

型號： HY-15763

起訂量：1

規(guī)格:：1 mg

價格:：￥500

我知道了

15906629305

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產(chǎn)品詳情

Erastin 是鐵誘導(dǎo)的細(xì)胞死亡 (ferroptosis) 誘導(dǎo)劑。Erastin 結(jié)合且抑制電壓依賴性陰離子通道 (VDAC2/VDAC3)。

生物活性

Erastin is a ferroptosis inducer. Erastin binds and inhibits voltage-dependent anion channels (VDAC2/VDAC3)^[1].

體外研究
(In Vitro)

Erastin triggers oxidative, iron-dependent cell death. Treatment of NRAS-mutant HT-1080 fibrosarcoma cells with the RSL molecule erastin (10 μM) results in a time-dependent increase in cytosolic and lipid ROS beginning at 2 hours^[1]. Cell death triggered by erastin is significantly inhibited by antioxidants (e.g., α-tocopherol, butylated hydroxytoluene, and β-carotene) and iron chelators, suggesting that ROS- and iron-dependent signaling is required for erastin-induced ferroptosis. Erastin can directly bind to VDAC2/3 in BJeLR cells. Knockdown of VDAC2 and VDAC3, but not VDAC1, leads to erastin resistance. Erastin has the ability to reduce glutathione level by directly inhibiting cystine/glutamate antiporter system Xc? activity, with activation of the ER stress response^[2]. Erastin potently inhibits HT-29 cell survival. Erastin shows a dose-dependent effect, and 30 μM of erastin displays the most dramatic effect^[3].

體內(nèi)研究
(In Vivo)

Intraperitoneal injection of erastin at well-tolerated doses dramatically inhibits HT-29 xenograft growth in severe combined immunodeficient mice^[3].

分子量

547.04

Formula

C₃₀H₃₁ClN₄O₄

CAS 號

571203-78-6

運輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

Powder	-20°C	3 years
	4°C	2 years

*該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。

溶解性數(shù)據(jù)

In Vitro:

DMSO : 12.5 mg/mL (22.85 mM; Need ultrasonic)

H₂O : < 0.1 mg/mL (insoluble)

配制儲備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	1.8280 mL	9.1401 mL	18.2802 mL
5 mM	0.3656 mL	1.8280 mL	3.6560 mL
10 mM	0.1828 mL	0.9140 mL	1.8280 mL

請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液；該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。

In Vivo:

——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

1.

請依序添加每種溶劑： 50% PEG300 50% saline

Solubility: 5 mg/mL (9.14 mM); Suspended solution; Need ultrasonic
2.

請依序添加每種溶劑： 10% DMSO 90% corn oil

Solubility: ≥ 1.25 mg/mL (2.29 mM); Clear solution
3.

請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% saline

Solubility: ≥ 1 mg/mL (1.83 mM); Clear solution

參考文獻(xiàn)

[1]. Dixon SJ, et al. Ferroptosis: an iron-dependent form of nonapoptotic cell death. Cell. 2012 May 25;149(5):1060-72.

[2]. Xie Y, et al. Ferroptosis: process and function. Cell Death Differ. 2016 Mar;23(3):369-79.

[3]. Huo H, et al. Erastin Disrupts Mitochondrial Permeability Transition Pore (mPTP) and Induces Apoptotic Death of Colorectal Cancer Cells. PLoS One. 2016 May 12;11(5):e0154605.

Erastin

Erastin 是鐵誘導(dǎo)的細(xì)胞死亡 (ferroptosis) 誘導(dǎo)劑。Erastin 結(jié)合且抑制電壓依賴性陰離子通道 (VDAC2/VDAC3)。
貨號： HY-15763

品牌：MCE

型號： HY-15763

起訂量：1

規(guī)格:：1 mg

價格:：￥500

15906629305

在線客服

客服一

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產(chǎn)品詳情

Erastin 是鐵誘導(dǎo)的細(xì)胞死亡 (ferroptosis) 誘導(dǎo)劑。Erastin 結(jié)合且抑制電壓依賴性陰離子通道 (VDAC2/VDAC3)。

生物活性

Erastin is a ferroptosis inducer. Erastin binds and inhibits voltage-dependent anion channels (VDAC2/VDAC3)^[1].

體外研究
(In Vitro)

體內(nèi)研究
(In Vivo)

Intraperitoneal injection of erastin at well-tolerated doses dramatically inhibits HT-29 xenograft growth in severe combined immunodeficient mice^[3].

分子量

547.04

Formula

C₃₀H₃₁ClN₄O₄

CAS 號

571203-78-6

運輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

Powder	-20°C	3 years
	4°C	2 years

*該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。

溶解性數(shù)據(jù)

In Vitro:

DMSO : 12.5 mg/mL (22.85 mM; Need ultrasonic)

H₂O : < 0.1 mg/mL (insoluble)

配制儲備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	1.8280 mL	9.1401 mL	18.2802 mL
5 mM	0.3656 mL	1.8280 mL	3.6560 mL
10 mM	0.1828 mL	0.9140 mL	1.8280 mL

In Vivo:

1.

請依序添加每種溶劑： 50% PEG300 50% saline

Solubility: 5 mg/mL (9.14 mM); Suspended solution; Need ultrasonic
2.

請依序添加每種溶劑： 10% DMSO 90% corn oil

Solubility: ≥ 1.25 mg/mL (2.29 mM); Clear solution
3.

請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% saline

Solubility: ≥ 1 mg/mL (1.83 mM); Clear solution